期刊
JOURNAL OF BIOTECHNOLOGY
卷 155, 期 3, 页码 287-292出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2011.07.014
关键词
Anti-microRNA oligonucleotides (AMOs); Gold nanoparticles; miR-29; MCL-1
资金
- Basic Science Research Program [2009-0066379]
- Ministry of Education, Science and Technology [2009-0093821]
- Pioneer Research Center [2008-2000122]
- National Research Foundation of Korea [2011-0001812, 2009-0093821, 과09A1215, 2009-0066320] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demonstrate the ability of AMOs to silence miRNA, we selected miR-29b, which is known to downregulate myeloid cell leukemia-1 (MCL-1), a factor responsible for promoting cell survival. We first generated AuNPs coated with cargo DNA, which was then coupled to complementary DNA linked to an antisense miR-29b sequence. When the AuNPs were delivered into HeLa cells, MCL-1 protein and mRNA levels were increased significantly. Furthermore, apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was inhibited, proving that AMOs targeting miR-29b were effectively delivered by our innovative AuNP. In addition, we provided evidence that AuNP could deliver other AMOs against miR-21 into two independent cell lines, KGN and 293T, suggesting that the AuNP conjugates can be versatile for any AMO and cell type.
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