期刊
JOURNAL OF BIOTECHNOLOGY
卷 150, 期 2, 页码 232-239出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2010.08.013
关键词
Perfluorotributylamine; beta TC-tet; Encapsulated cells; Normoxia; Hypoxia
资金
- ERC [EEC-9731643]
- NIH [DK47858, DK73991, DK76801]
- U S Department of Education through the Center for Drug Discovery Development and Delivery
Due to the high solubility of oxygen in perfluorocarbons (PFCs) these compounds have been explored for Improved cell and tissue oxygenation The goal of this study is to investigate the effects of a PFC emulsion on cellular growth and function in a tissue engineered construct A perfluorotributylamine (PFTBA) emulsion was co-encapsulated at 10 vol% with mouse beta TC-tet insulinoma cells in calcium alginate beads and cultured under normoxic and severely hypoxic conditions The number of metabolically active cells and the Induced insulin secretion rate were measured over time for up to 16 days Results showed no significant effect of PFTBA relative to the PFTBA-free control The alginate-PFC-cell system was also modeled mathematically and simulations tracked the number of viable cells over time under the same conditions used experimentally Simulations revealed only a small likely experimentally undetectable difference in cell density between the PFC-containing and PFC-free control beads It is concluded that PFTBA up to 10 vol% has no significant effect on the growth and function of encapsulated beta TC-tet cells under normoxic and hypoxic conditions (C) 2010 Elsevier B V All rights reserved
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