期刊
JOURNAL OF BIOTECHNOLOGY
卷 150, 期 1, 页码 171-174出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2010.07.013
关键词
Magnetic nanoparticals; Amino-silane; Immobilization; Lipase; Kinetic
资金
- Committee of Natural Science Foundation in China [J0730425]
- Opening Foundation of the State Key Laboratory for Oxo Synthesis and Selective Oxidation Open Foundation [OSSO0602]
- Opening Foundation of the Key Laboratory for Magnetism and Magnetic Material of the Education Ministry of China in Lanzhou Universit [MMM200825]
- Key Research Program of Gansu Province [2GS064-A52-036-02]
In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process. (C) 2010 Elsevier B.V. All rights reserved.
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