Facile synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and application for lipase immobilization

In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process. (C) 2010 Elsevier B.V. All rights reserved.