期刊
JOURNAL OF BIOTECHNOLOGY
卷 139, 期 3, 页码 236-249出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2008.12.004
关键词
Artificial organs; Bio-artificial pancreas; Hollow fibers in vascular device; Islets of Langerhans; beta-TC-3 cells; BAP-in vitro assays
资金
- Fundacao para a Ciencia Tecnologia [SFRH/BD/10594/2002]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/10594/2002] Funding Source: FCT
A system for vascular hollow fiber bio-artificial pancreas development, optimization and in vitro testing was implemented and operated in a simple and fully described manner, allowing other researchers to test a variety of experimental conditions (different biomaterials, biologic tissue, addition of proteins or other adjuvants). In this work, a polysulfone hollow fiber was used as bioprotective material. Two different cell sources were co-immobilized with agarose microspheres in and experimented with the membrane device: rat islets of Langerhans and mouse beta-TC-3 insulinoma cells. The results obtained with islets of Langerhans were used as islet comparable insulin-release data. Beta-TC-3 cells were mainly used in these studies, due to higher control and reproducibility of cell number and behavior: addition of hemoglobin was beneficial for sustained cell viability, especially during cell insertion in the device (viability assessed by beta-TC-3 lactate dehydrogenase activity in. the recirculating culture medium); cells did not adhere to the polysulfone membrane (assessed by SEM observation of membrane samples from dynamic cultures). Comparable device functionality and insulin-release results were attained with both cell types: device functionality was maintained for 7-9 days and maximum insulin-release during dynamic glucose challenges were 2.6 x 10(-3) +/- 7 x 10(-5) mu U/beta-cell x 8 h, with islets, and 9.3 x 10(-4) +/- 2 x 10(-5) mu U/beta-cell x 2 h, with beta-TC-3 cells. (C) 2008 Elsevier B.V. All rights reserved.
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