期刊
JOURNAL OF BIOTECHNOLOGY
卷 139, 期 1, 页码 124-129出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2008.08.008
关键词
Feruloyl esterase; Ionic liquids; Glycerol sinapate; LDL oxidative modification
The ability of a feruloyl esterase (AnFaeA). either in free or immobilised (cross-linked enzyme aggregates) form, to catalyse the esterification of glycerol, a major by-product of the biodiesel industry, with sinapic acid was studied in four hexafluorophosphate anion-containing ionic liquids: ((Bmiml[PF6], [Omim][PF6], [C(2)OHmim][PF6] and [C(5)O(2)mim][PF6]). Such ionic liquids are considered 'green' reaction systems. The synthetic reaction was optimised in [C(2)OHmim][PF6] and the highest conversion yield was 72.5 +/- 2.1%, while, at the same reaction conditions in [C(5)O(2)mim] [PF6], a similar conversion yield was obtained (76.7 +/- 1.5%). AnFaeA was active in its free and immobilised form, with the latter retaining a part of its synthetic activity after 5 consecutive 24 h-period reaction cycles. Sinapic acid was esterified to one of the primary hydroxyl groups of glycerol and retained, after esterification, 63.1 +/- 0.3% and 89.5 +/- 1.1% of its antioxidant activity against low-density lipoprotein oxidation, when added at concentrations of 10 and 60 mu M, respectively, in the assay mixture. (c) 2008 Elsevier B.V. All rights reserved.
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