期刊
JOURNAL OF BIOTECHNOLOGY
卷 139, 期 1, 页码 84-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2008.09.007
关键词
Ginkgo biloba; Bilobalide; Ginkgolide A; Ginkgolide B; Terpenoids; Cell suspension culture; Biotic elicitor
资金
- Regional Forest Research Foundation Works
- Korean Government (MOEHRD, Basic Research Promotion Fund) [KRF-2006-521-F00023]
The effect of biotic elicitors on the production of bilobalide and ginkgolides in Ginkgo biloba cell suspension cultures was studied. The treatment of cell cultures with Candida albicans and Staphylococcus aureus as elicitors increased the amounts of bilobalide (BB), ginkgolide A (GA) and ginkgolide B (GB), with slight growth inhibition. The native bacterial elicitor was more effective for secondary metabolite accumulations both in cells and culture medium than autoclaved. However, exposure times of the cells to the elicitors strongly influenced the production of BB, GA and GB. This study suggests that biotic elicitors can regulate the production of BB, GA and GB either directly or indirectly. These results also describe the establishment of optimum conditions that determine the effects of biotic elicitors on secondary metabolism of bilobalides. Crown Copyright (c) 2008 Published by Elsevier B.V. All rights reserved.
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