期刊
JOURNAL OF BIOTECHNOLOGY
卷 138, 期 3-4, 页码 67-73出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2008.07.1995
关键词
Sedimentation field-flow fractionation; Inclusion bodies; beta-Lactamase; Fed-batch cultivation
资金
- Akademisch Soziale Arbeitsgemeinschaft Osterreichs (ASAG)
- Austrian Science Fund FWF [1.191-1310]
- Austrian Center of Biopharmaceutical Technology (ACBT)
Sedimentation field-flow fractionation (sedFFF) was evaluated to characterize the size of Delta(4-23)TEM-beta-lactamase inclusion bodies (IBs) overexpressed in fed-batch cultivations of Escherichia coli. Heterologous Delta(4-23)TEM-beta-lactamase protein formed different sizes of IBs, depending upon the induction conditions. In the early phases of recombinant protein expression, induced with low concentrations of IPTG (isopropyl-beta-D-thiogalactoside), 113 masses were larger than expected and showed heterogeneous size distributions. During cultivation, IB sizes showed a Gaussian distribution and reached a broad range by the end of the fed-batch cultivations. The obtained result proved the aptitude of sedFFF to rapidly assess the size distribution of IBs in a culture. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据