期刊
NEURAL REGENERATION RESEARCH
卷 10, 期 7, 页码 1082-1087出版社
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/1673-5374.160099
关键词
nerve regeneration; middle cerebral artery occlusion; cerebral ischemia; cytotoxic edema; angioedema; magnetic resonance imaging; diffusion-weighted imaging; aquaporin-4; gene silencing
资金
- National Natural Science Foundation of China [30960399]
- Hainan Provincial International Cooperation Project of China [Qiongke(2012)65]
- Hainan Provincial Health Department Project of China [2011-SWK-10-136/Qiongwei2011-65]
Aquaporin-4 regulates water molecule channels and is important in tissue regulation and water transportation in the brain. Upregulation of aquaporin-4 expression is closely related to cellular edema after early cerebral infarction. Cellular edema and aquaporin-4 expression can be determined by measuring cerebral infarct area and apparent diffusion coefficient using diffusion-weighted imaging (DWI). We examined the effects of silencing aquaporin-4 on cerebral infarction. Rat models of cerebral infarction were established by occlusion of the right middle cerebral artery and siRNA-aquaporin-4 was immediately injected via the right basal ganglia. In control animals, the area of high signal intensity and relative apparent diffusion coefficient value on T2-weighted imaging (T2WI) and DWI gradually increased within 0.5-6 hours after cerebral infarction. After aquaporin-4 gene silencing, the area of high signal intensity on T2WI and DWI reduced, relative apparent diffusion coefficient value was increased, and cellular edema was obviously alleviated. At 6 hours after cerebral infarction, the apparent diffusion coefficient value was similar between treatment and model groups, but angioedema was still obvious in the treatment group. These results indicate that aquaporin-4 gene silencing can effectively relieve cellular edema after early cerebral infarction; and when conducted accurately and on time, the diffusion coefficient value and the area of high signal intensity on T2WI and DWI can reflect therapeutic effects of aquaporin-4 gene silencing on cellular edema.
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