期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 117, 期 2, 页码 165-169出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2013.07.007
关键词
Furfural; TAL1; ADH1; Overexpression; Hemicellulosic hydrolysate; Xylose; Saccharomyces cerevisiae; Bioethanol
资金
- New Energy and Industrial Technology Development Organization (NEDO) [P07015]
- Ministry of Economy, Trade, and Industry (METI) of Japan
- Ministry of Higher Education of Malaysia
- University Malaysia Perlis (UniMAP), Malaysia
Lignocellulosic biomass dedicated to bioethanol production usually contains pentoses and inhibitory compounds such as furfural that are not well tolerated by Saccharomyces cerevisiae. Thus, S. cerevisiae strains with the capability of utilizing both glucose and xylose in the presence of inhibitors such as furfural are very important in industrial ethanol production. Under the synergistic conditions of transaldolase (TAL) and alcohol dehydrogenase (ADH) overexpression, S. cerevisiae MT8-1X/TAL ADH was able to produce 1.3-fold and 2.3-fold more ethanol in the presence of 70 mM furfural than a TAL-expressing strain and a control strain, respectively. We also tested the strains' ability by mimicking industrial ethanol production from hemicellulosic hydrolysate containing fermentation inhibitors, and ethanol production was further improved by 16% when using MT8-1X/TAL-ADH compared to the control strain. Transcript analysis further revealed that besides the pentose phosphate pathway genes TKL1 and TAL1, ADH7 was also upregulated in response to furfural stress, which resulted in higher ethanol production compared to the TAL-expressing strain. The improved capability of our modified strain was based on its capacity to more quickly reduce furfural in situ resulting in higher ethanol production. The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
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