期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 115, 期 5, 页码 490-496出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.11.009
关键词
beta-Glucosidase; Thermofilum pendens; Cellulose; Glucoside; Archaeon
资金
- National Natural Science Foundation of China [31000760]
- program for New Century Excellent Talents in University from Ministry of Education of China [NCET-11-0982]
- Globalization of Korean Foods R D program [911038-1]
- Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea
The gene encoding a beta-glucosidase from the archaeon Thermofilum pendens (Tpbgl) was cloned and expressed in Escherichia coli. The purified recombinant enzyme had a molecular mass of 77.8 kDa and released glucose or mannose from p-nitrophenyl-beta-D-glucopyra (pNPG), cellobiose, mannobiose, and genistin. Peak Tpbgl activity was detected at 90 degrees C, and 50% activity remained after incubation for 60 min at 95 degrees C. The optimal pH for pNPG hydrolysis was 3.5. When the enzyme was incubated with pNPG in the presence of ethanol and propanol, the glucose moiety was transferred to acceptor alcohols. Tpbgl is the archaeal beta-glucosidase from glucoside hydrolase family 3 and found to be most heat stable under extremely acidic conditions (pH 3.5). The kinetic parameters revealed that Tpbgl had the highest catalytic efficiency toward pNPG (k(cat)/K-m = 3.05) with strong substrate affinity for such natural substrates as cellobiose (K-m = 0.149) and mannobiose (K-m = 0.147). Genistin solubilized in 10-40% DMSO was hydrolyzed to genistein with nearly 99% conversion, indicating that high concentrations of the water-insoluble isoflavone glycoside can be treated by the enzyme. Our results indicate that Tpbgl has great potential in cellulose saccharification and the glucoside hydrolysis of natural compounds. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.
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