期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 115, 期 6, 页码 654-657出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.12.012
关键词
Bacillus subtilis; Rifampicin resistance; Colored soybean; Exoenzyme production; Natto
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [221S0002]
- Grants-in-Aid for Scientific Research [221S0002] Funding Source: KAKEN
The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the beta-subunit of the RNA polymerase. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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