期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 114, 期 6, 页码 630-634出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.07.004
关键词
Microcystin biodegradation pathway; Microcystin; Microcystin-degrading bacterium; mlr gene cluster; MlrB; MlrC
资金
- Japan Society for the Promotion of Science (JSPS) [21310049]
- JSPS [24710081]
- Grants-in-Aid for Scientific Research [24710081, 21310049] Funding Source: KAKEN
The Ink gene cluster consisting of mlrA, mlrB, mlrC, and mlrD is involved in the degradation of the cyanobacterial toxin microcystin. However, it is unclear which degradation intermediates are metabolized by MlrB and MlrC. To address these questions, we constructed recombinant Escherichia coli to overproduce MlrB and MlrC from Sphingopyxis sp. C-1, and determined which intermediates were degraded in cell-free extracts. The cell-free extract containing MlrB degraded linearized microcystin-LR, giving rise to a tetrapeptide. The cell-free extract of MlrC degraded linearized microcystin-LR and also degraded the tetrapeptide to the amino acid Adda. These results indicate that linearized microcystin-LR is degraded by both MlrB and MlrC, and tetrapeptide is degraded by specifically by MlrC in Sphingopyxis sp. C-1. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据