期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 114, 期 3, 页码 262-267出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.04.006
关键词
Brefeldin A; Identification; Eupenicillium brefeldianum; Breeding; Fermentation
资金
- 973 Program [2011CB710800]
- Major Program for Key Science and Technology of Zhejiang Province of China [2009C13G2020012]
- Natural Science Foundation of Zhejiang Province of China [Y4090605]
In this work, a robust brefeldin A-synthesizing fungus, Eupenicillium brefeldianum ZJB082702, was bred from a Murraya paniculata endophytic fungus E. brefeldianum A1163. Using one-factor-at-a-time experimental design, optimization of media composition for E. brefeldianum ZJB082702 fermenting brefeldin A was conducted. Outcomes indicated that mixed carbon source and mixed nitrogen source were of critical importance to brefeldin A fermentation. After 6 d culture in the optimized fermentation media, composed of (gl(-1)) 13.33 starch, 26.67 glucose, 1.0 yeast extract powder, 1.0 corn steep liquor, 0.5 soybean meal, 0.75 NaNO3, 2.5 malt extract, 6.0 CaCO3, 3.0 MgSO4, 4.0 KH2PO4, 1.0 x 10(-2) CuSO4, brefeldin A yield peaked at 1304.7 mg l(-1), 648.2 mg l(-1) in 500 ml baffled flask and 15 l stirred fermentor respectively, formed as a growth associated type of secondary metabolite based on fermentation profile analysis. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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