期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 113, 期 6, 页码 697-703出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.01.016
关键词
Trichoderma reesei; Laccase; Trametes sp AH28-2; Heterologous expression; Biomass conversion
资金
- National Basic Research Program of China [2011CB707403]
- National Natural Science Foundation of China [31030001, 30970026, 30800024]
The Trametes sp. AH28-2 laccase gene lacA fused to cellobiohydrolase 1 signal peptide coding sequence was heterologously expressed in T. reesei. The lacA cDNA was under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter. Native PAGE analysis indicated that two transformants, L8 and 138, were able to secrete recombinant laccase A, and their laccase activities corresponding to ABTS oxidation reached 3.62 IU ml(-1) and 1.50 IU ml(-1) respectively. Most of the characteristics of the recombinant laccase were similar to those of the native enzyme. Reducing sugar yields of L8 and 138 obtained from saccharification of corn residue by crude enzyme increased by 31.3% and 71.6% respectively compared to the host strain. These results indicated that the engineering strains developed in this work could be potentially used for laccase production and tailoring cellulase properties with laccase proteins through genetic manipulation would be a feasible strategy to improve saccharification efficiency of biomass by cellulase preparation. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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