期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 114, 期 3, 页码 334-341出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.04.005
关键词
Fruit size; Pumpkin; Gigantism; Protein expression pattern; Two-dimensional gel electrophoresis; Cell size; DNA content
资金
- Sojo University
- Grants-in-Aid for Scientific Research [22580109, 23790370] Funding Source: KAKEN
Common pumpkin plants (Cucurbita maxima) produce fruits of 1-2 kg size on the average, while special varieties of the same species called Atlantic Giant are known to produce a huge fruit up to several hundred kilograms. As an approach to determine the factors controlling the fruit size in C. maxima, we cultivated both AG and control common plants, and found that both the cell number and cell sizes were increased in a large fruit while DNA content of the cell did not change significantly. We also compared protein patterns in the leaves, stems, ripe and young fruits by two-dimensional (2D) gel electrophoresis, and identified those differentially expressed between them with mass spectroscopy. Based on these results, we suggest that factors in photosynthesis such as ribulose-bisphosphate carboxylase, glycolysis pathway enzymes, heat-shock proteins and ATP synthase play positive or negative roles in the growth of a pumpkin fruit. These results provide a step toward the development of plant biotechnology to control fruit size in the future. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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