期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 107, 期 3, 页码 324-330出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2008.10.014
关键词
Peptide array; Piezoelectric ceramic micropump; Cow's milk allergy; IgE-binding epitopes; spotting buffer
资金
- Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government
Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
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