期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 108, 期 1, 页码 1-4出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2009.02.007
关键词
Cell-free protein synthesis; Energy source; ATP; Cell extract; Glycolysis
The productivity of cell-free protein synthesis depends critically upon the efficiency of ATP regeneration. In addition, the expensive energy sources used in the conventional cell-free protein synthesis systems account for the major part of the overall cost for the expression of proteins. Therefore, it is essential to implement a cheaper and more efficient ATP regeneration method in order to make cell-free protein synthesis a viable option for industrial protein production. In this article, we review the recent progress in the methodologies for supplying ATP during cell-free protein synthesis in E coli extracts. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
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