期刊
JOURNAL OF BIOPHOTONICS
卷 7, 期 8, 页码 617-623出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201300010
关键词
M13 bacteriophage; optical imaging; fluorescent dye; bacterial infections
资金
- National Cancer Institute Center for Cancer Nanotechnology Excellence [5-U54-CA151884-03]
We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo. We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can target and distinguish pathogenic infections of F-pili expressing and F-negative strains of E. coli. Further, in order to tune this M13-Dye complex suitable for targeting other strains of bacteria, we have used a 1-step reaction for creating an anti-bacterial antibody-M13-Dye probe. As an example, we show anti-S. aureus-M13-Dye able to target and image infections of S. aureus in living hosts, with a 3.7 x increase in fluorescence over background.
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