期刊
JOURNAL OF BIOPHOTONICS
卷 5, 期 5-6, 页码 437-448出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200003
关键词
skin; tissue engineering; microscopy; functional imaging; multiphoton fluorescence microscopy; fluorescence lifetime imaging microscopy; optical coherence tomography
资金
- National Science Foundation [CBET 08-52658 ARRA, CBET 10-33906]
- National Institutes of Health Environmental Health Sciences
- Developmental and Reproductive Toxicology at the University of Illinois at Urbana-Champaign
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1033906, 0852658] Funding Source: National Science Foundation
An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enabling simultaneous visualization of multiple contrast sources and mechanisms from cells and tissues. Spatially co-registered OCM/MPM/FLIM images of multi-layered skin tissues are obtained, which are formed based on complementary information provided by different modalities, i.e., scattering information from OCM, molecular information from MPM, and functional cellular metabolism states from FLIM. Cellular structures in both the dermis and epidermis, especially different morphological and physiological states of keratinocytes from different epidermal layers, are revealed by mutually-validating images. In vivo imaging of human skin is also investigated, which demonstrates the potential of multimodal microscopy for in vivo investigation during engineered skin engraftment. This integrated imaging technique and microscope show the potential for investigating cellular dynamics in developing engineered skin and following in vivo grafting, which will help refine the control and culturing conditions necessary to obtain more robust and physiologically-relevant engineered skin substitutes. (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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