期刊
JOURNAL OF BIOPHOTONICS
卷 7, 期 5, 页码 312-322出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201200135
关键词
biochemical sensing; long period grating; flow cell; label-free; immunoassay
Optical fibre gratings, especially long period gratings, have been recently proposed as optical devices for biochemical sensing. A biochemical interaction along the grating portion induces a refractive index change and hence a change in the fiber transmission spectrum. This provides an alternative methodology with respect to other label-free optical approaches, such as surface plasmon resonance, interferometric configurations and optical resonators. The fibre biofunctionalization has been carried out by means of a novel chemistry using Eudragit L100 copolymer as opposed to the commonly used silanization procedure. Antigen-antibody interaction has been analysed by means of an IgG/anti-IgG bioassay. The biosensor was fully characterised, monitoring the kinetics during the antibody immobilization and the antigen interaction and achieving the calibration curve of the assay. A comparison of the biosensor performance was made by using two different long period gratings with distinct periods. ((c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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