期刊
JOURNAL OF BIOPHOTONICS
卷 5, 期 10, 页码 754-767出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201100119
关键词
Cy5; 5; Lycopersicon esculentum lectin; mouse; fluorescence microscopy; cardiac imaging techniques; endothelial cells
资金
- Manitoba Health Research Council
- Heart and Stroke Foundation of Manitoba
- Manitoba Graduate Scholarship
Cy5.5-lectin, a non-toxic conjugate, combines the benefits of near-infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC-lectin and ICAM2 antibodies, Cy5.5-lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real-time and post-infusion. Ex-vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in-vivo tail-vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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