期刊
JOURNAL OF BIOPHOTONICS
卷 4, 期 11-12, 页码 805-813出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201100008
关键词
melanosomes; Raman; photodamage; light irradiation
资金
- NIH [R03EB008535, R15EY020805, R01EY013722, P30EY01931]
- NSF [DBI-0964225]
- Research to Prevent Blindness, Inc
Melanosomes are long lived organelles in retinal pigment epithelium cells and are primarily responsible for photoprotection. However, with aging or prolong light radiation, the function of melanosomes diminishes, which may be due to photobleaching of melanin pigments present in melanosomes. In this study, melanosomes were isolated from the retinal pigment epithelium cells and exposed to green light (532 nm), and the chemical changes were monitored using Raman microspectroscopy. Photochemical changes were recorded for different power levels and exposure times. The threshold power and the rate for irreversible photobleaching of melanosomes were calculated by fitting the experimental data with a proposed model.
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