期刊
JOURNAL OF BIOPHOTONICS
卷 3, 期 4, 页码 216-223出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200900102
关键词
optical tweezers; micromanipulation; HIV-1; cell-cell transmission; fluorescence microscopy
资金
- NIH [AI074420-02]
- Burroughs Wellcome Fund
- Hirschl Weill-Caulier Career Scientist Award
- NSF Center for Biophotonics Science and Technology [PHY012099]
- UC Davis Health System Research award
- UCD CTSC (NCRR) [ULRR024146]
Cell-cell interactions through direct contact are very important for cellular communication and coordination especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer. [GRAPHICS] HIV-1 infected T cell (green) decorated with uninfected primary T cells (red) by manipulating the primary cells with an optical tweezers system.
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