He-Ne baser 632.8 nm pre-irradiation gives protection against DNA damage induced by a near-infrared trapping beam

We report the results of a study carried out to investigate the effect of He-Ne laser (632.8 nm) pre-irradiation on DNA damage induced by continuous wave 1064 nm trapping beam exposure in MCF-7 cells. A significant decrease in % tail DNA (p < 0.05) was observed in MCF-7 cells pre-exposed to He-No laser beam. The dependence of the induced protection against 1064 nm trapping beam irradiation induced DNA damage on the time interval between the two irradiations as well as the He-Ne laser pre-irradiation parameters is presented. Presence of DNA in tail of Comet, the assay used for monitoring DNA damage caused by high power (250 mW) near infrared trapping beam and low level He-Ne laser pre-irradiation induced protection.