期刊
JOURNAL OF BIOPHOTONICS
卷 3, 期 5-6, 页码 368-372出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200900082
关键词
photodynamic therapy; microRNA; apoptosis; HeLa cells; talaporfin sodium
资金
- Grants-in-Aid for Scientific Research [21687011] Funding Source: KAKEN
Apoptosis-related microRNAs (miRNAs), the expression levels of which were observed to increase after photodynamic therapy (PDT), were identified as markers of PDT efficacy. miRNAs form a class of small endogenous RNAs that play important regulatory roles by targeting mRNA for cleavage or translational repression. miRNAs contribute to diverse biological processes, including development, cell growth, apoptosis, and hematopoiesis, which suggests a role in cancer development. However, no previous reports have described a relationship between apoptosis and miRNA expression after PDT. In this study, apoptosis-related miRNA levels were characterized by real-time PCR after PDT in He La cells. miR-210 and miR-296 expression levels increased 1 hour after PDT, although the other apoptosis-related miRNA (miR-7, miR-148a, miR-204, and miR-216) expression levels were unchanged. Since miR-210 is the most prominent miRNA consistently stimulated under hypoxic conditions and VEGF is capable of increasing miR-296 expression levels, these data suggest that hypoxia induced by PDT induces miR-210 expression, followed by an increase of VEGF expression and miR-296 expression. These results constitute the first report of an analysis of miRNA expression after PDT using talaporfin sodium as a photosensitizer, demonstrating that miR-210 and miR-296 expression levels are markers of PDT efficacy in HeLa cells.
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