期刊
JOURNAL OF BIOPHOTONICS
卷 1, 期 6, 页码 478-493出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.200810058
关键词
Multi-photon tomography; fluorescence lifetime imaging; pharmacokinetics; zinc oxide nanoparticle; fluorescein
Multiphoton tomography was used to examine xenobiotic transport in vivo. We used the photochemical properties of zinc oxide and fluorescein and multiphoton tomography to study their transport in the skin and in the rat liver in vivo. Zinc oxide nanoparticles were visualised in human skin using the photoluminescence properties of zinc oxide and either a selective emission wavelength band pass filter or a filter with fluorescence lifetime imaging (FLIM). Zinc oxide nanoparticles (30 nm) did not penetrate into human skin in vitro and in vivo and this was validated by scanning electron microscopy with Xray photoelectron spectroscopy. Fluorescein was measured in the liver using FLIM. Fluorescein is rapidly extracted from the blood into the liver cells and then transported into the bile. It is suggested that multipho- [GRAPHICS] ton tomography may be of particular use in defining in vivo 4D (in both space and time) pharmacokinetics. (c) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据