期刊
JOURNAL OF BIOMOLECULAR NMR
卷 59, 期 1, 页码 15-22出版社
SPRINGER
DOI: 10.1007/s10858-014-9820-8
关键词
Solid-state NMR; Magic-angle spinning; BSH-CP; Protein resonance assignment; Ubiquitin
资金
- Max Planck Society
- DFG
- European Union [261863]
- MPG-CAS
- EMBO
We have recently presented band-selective homonuclear cross-polarization (BSH-CP) as an efficient method for CO-CA transfer in deuterated as well as protonated solid proteins. Here we show how the BSH-CP CO-CA transfer block can be incorporated in a set of three-dimensional (3D) solid-state NMR (ssNMR) pulse schemes tailored for resonance assignment of proteins at high static magnetic fields and moderate magic-angle spinning rates. Due to the achieved excellent transfer efficiency of 33 % for BSH-CP, a complete set of 3D spectra needed for unambiguous resonance assignment could be rapidly recorded within 1 week for the model protein ubiquitin. Thus we expect that BSH-CP could replace the typically used CO-CA transfer schemes in well-established 3D ssNMR approaches for resonance assignment of solid biomolecules.
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