期刊
JOURNAL OF BIOMOLECULAR NMR
卷 57, 期 4, 页码 319-326出版社
SPRINGER
DOI: 10.1007/s10858-013-9795-x
关键词
Ultracentrifuge; Sedimentation; Solid state NMR; Biological macromolecules; Aggregates
资金
- EC [228461, 261572, 261863, 211252]
- project PRIN Biologia strutturale meccanicistica: avanzamenti metodologici e biologici'' [2009FAKHZT_001]
- Ente Cassa Risparmio Firenze
We have proposed solid state NMR (SSNMR) of sedimented solutes as a novel approach to sample preparation for biomolecular SSNMR without crystallization or other sample manipulations. The biomolecules are confined by high gravity-obtained by centrifugal forces either directly in a SSNMR rotor or in a ultracentrifugal device- into a hydrated non-crystalline solid suitable for SSNMR investigations. When gravity is removed, the sample reverts to solution and can be treated as any solution NMR sample. We here describe a simple web tool to calculate the relevant parameters for the success of the experiment.
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