期刊
JOURNAL OF BIOMOLECULAR NMR
卷 52, 期 4, 页码 329-337出版社
SPRINGER
DOI: 10.1007/s10858-012-9613-x
关键词
Intrinsically disordered proteins; Non-uniform sampling; Backbone assignment; 5D NMR; 6D NMR
资金
- EC [261863]
- Foundation for Polish Science
- EU
- Czech Science Foundation [204/09/0583]
- [AV0Z50200510]
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.
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