4.3 Article

A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies

期刊

JOURNAL OF BIOMOLECULAR NMR
卷 50, 期 3, 页码 229-236

出版社

SPRINGER
DOI: 10.1007/s10858-011-9513-5

关键词

NMR spectroscopy; High molecular weight proteins; Assignment; Site-specific mutagenesis; Methyl-group labeling

资金

  1. CONACYT
  2. CNRS
  3. Agence Nationale de la Recherche [ANR-09-PIRIBio-445583, ANR-07-BLAN-0085]
  4. Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0085] Funding Source: Agence Nationale de la Recherche (ANR)

向作者/读者索取更多资源

Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance assignment of methyl probes in high molecular weight protein assemblies can be efficiently achieved by combining fast NMR experiments, residue-type-specific isotope-labeling and automated site-directed mutagenesis. The utility of this general and straightforward strategy is demonstrated through the characterization of intermolecular interactions involving a 468-kDa multimeric aminopeptidase, PhTET2.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据