期刊
JOURNAL OF BIOMOLECULAR NMR
卷 47, 期 2, 页码 125-134出版社
SPRINGER
DOI: 10.1007/s10858-010-9413-0
关键词
Carbohydrates; Polysaccharide; Biopolymer; NMR spectroscopy; Absolute configuration; Dynamic light scattering
资金
- Swedish Research Council
- The Knut and Alice Wallenberg Foundation
- Carl Tryggers Stiftelse for Vetenskaplig Forskning
- Magn. Bergvalls Stiftelse
- Academy of Finland [210653]
- Sino-Finnish Cooperative Project [2006DFA32620]
The use of lactic acid bacteria in fermentation of milk results in favorable physical and rheological properties due to in situ exopolysaccharide (EPS) production. The EPS from S. thermophilus ST1 produces highly viscous aqueous solutions and its structure has been investigated by NMR spectroscopy. Notably, all aspects of the elucidation of its primary structure including component analysis and absolute configuration of the constituent monosaccharides were carried out by NMR spectroscopy. An array of techniques was utilized including, inter alia, PANSY and NOESY-HSQC TILT experiments. The EPS is composed of hexasaccharide repeating units with the following structure: -> 3)[alpha-d-Glcp-(1 -> 4)]-beta-d-Galp-(1 -> 4)-beta-d-Glcp-(1 -> 4)[beta-d-Galf-(1 -> 6)]-beta-d-Glcp-(1 -> 6)-beta-d-Glcp-(1 ->, in which the residues in square brackets are terminal groups substituting backbone sugar residues that consequently are branch-points in the repeating unit of the polymer. Thus, the EPS consists of a backbone of four sugar residues with two terminal sugar residues making up two side-chains of the repeating unit. The molecular mass of the polymer was determined using translational diffusion experiments which resulted in M(w) = 62 kDa, corresponding to 64 repeating units in the EPS.
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