期刊
JOURNAL OF BIOMOLECULAR NMR
卷 46, 期 1, 页码 89-100出版社
SPRINGER
DOI: 10.1007/s10858-009-9365-4
关键词
Site-specific labeling; Unnatural amino acids; Spin label; Metal chelator; In-cell NMR
A large number of amino acids other than the canonical amino acids can now be easily incorporated in vivo into proteins at genetically encoded positions. The technology requires an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is added to the media while a TAG amber or frame shift codon specifies the incorporation site in the protein to be studied. These unnatural amino acids can be isotopically labeled and provide unique opportunities for site-specific labeling of proteins for NMR studies. In this perspective, we discuss these opportunities including new photocaged unnatural amino acids, outline usage of metal chelating and spin-labeled unnatural amino acids and expand the approach to in-cell NMR experiments.
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