期刊
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
卷 -, 期 -, 页码 -出版社
HINDAWI PUBLISHING CORPORATION
DOI: 10.1155/2012/405842
关键词
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资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology (MEST) [NRF-2009-C1AAA001-2009-0093479, NRF-2009-0076912, NRF-2010-0006436, R32-2008-000-20054-0]
- Intelligent Synthetic Biology Center of Global Frontier Project
- MEST [2011-0031948]
- Next-Generation BioGreen 21 Program (SSAC) [PJ0099058]
- Rural Development Administration, Republic of Korea
- UNIST (Ulsan National Institute of Science and Technology) research grant
A major technical challenge in the cost-effective production of cellulosic biofuel is the need to lower the cost of plant cell wall degrading enzymes (PCDE), which is required for the production of sugars from biomass. Several competitive, low-cost technologies have been developed to produce PCDE in different host organisms such as Escherichia coli, Zymomonas mobilis, and plant. Selection of an ideal host organism is very important, because each host organism has its own unique features. Synthetic biology-aided tools enable heterologous expression of PCDE in recombinant E. coli or Z. mobilis and allow successful consolidated bioprocessing (CBP) in these microorganisms. In-planta expression provides an opportunity to simplify the process of enzyme production and plant biomass processing and leads to self-deconstruction of plant cell walls. Although the future of currently available technologies is difficult to predict, a complete and viable platform will most likely be available through the integration of the existing approaches with the development of breakthrough technologies.
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