期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 18, 期 10, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.18.10.101316
关键词
photoacoustic tomography; Forster resonance energy transfer; deep imaging; Forster resonance energy transfer efficiency
资金
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959, DP1 EB016986]
Forster resonance energy transfer (FRET) is a distance-dependent process that transfers excited state energy from a donor molecule to an acceptor molecule without the emission of a photon. The FRET rate is determined by the proximity between the donor and the acceptor molecules; it becomes significant only when the proximity is within several nanometers. Therefore, FRET has been applied to visualize interactions and conformational changes of biomolecules, such as proteins, lipids, and nucleic acids that cannot be resolved by optical microscopy. Here, we report photoacoustic tomography of FRET efficiency at a 1-cm depth in chicken breast tissue, whereas conventional high-resolution fluorescence imaging is limited to < 0.1 cm. Photoacoustic tomography is expected to facilitate the examination of FRET phenomena in living organisms. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
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