期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 17, 期 6, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.6.060506
关键词
photoacoustic microscopy; myocardial sheet architecture; label-free; undehydrated
资金
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959]
Cardiac myofibers are organized into sheet architectures, which contribute to up to 40% of the heart wall thickening for ejection of blood for circulation. It is important to delineate the sheet architecture for a better understanding of cardiac mechanisms. However, current sheet imaging technologies are limited by fixation-induced dehydration/deformation and low spatial resolution. Here we implemented high-resolution label-free photoacoustic microscopy (PAM) of the myocardial sheet architecture. With high endogenous optical-absorption contrast originating mainly from cytochrome, myoglobin, and melanin, PAM can image the unfixed, unstained and unsliced heart without introducing deformation artifacts. A fresh blood-free mouse heart was imaged by PAM ex vivo. The three-dimensional branching sheets were clearly identified within 150 mu m depth. Various morphological parameters were derived from the PAM image. The sheet thickness (80 +/- 10 mu m) and the cleavage height (11 +/- 1 mu m) were derived from an undehydrated heart for the first time. Therefore, PAM has the potential for the functional imaging of sheet architecture in ex vivo perfused and viable hearts. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.6.060506]
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