期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 17, 期 5, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.5.057006
关键词
blood microparticles; light scattering; inverse light-scattering problem; scanning flow cytometry; global optimization; Mie theory
资金
- program of Presidium of the Russian Academy of Science [2009-27-15]
- program of the Russian Government Research and educational personnel of innovative Russia [P1039, P422, 14.740.11.0729, 14.740.11.0921]
- Government of Russian Federation [11.G34.31.0034]
- Program of the Russian Federation for State Support of the Leading Scientific Schools [NSh-65387.2010.4]
We describe a novel approach to study blood microparticles using the scanning flow cytometer, which measures light scattering patterns (LSPs) of individual particles. Starting from platelet-rich plasma, we separated spherical microparticles from non-spherical plasma constituents, such as platelets and cell debris, based on similarity of their LSP to that of sphere. This provides a label-free method for identification (detection) of microparticles, including those larger than 1 mu m. Next, we rigorously characterized each measured particle, determining its size and refractive index including errors of these estimates. Finally, we employed a deconvolution algorithm to determine size and refractive index distributions of the whole population of microparticles, accounting for largely different reliability of individual measurements. Developed methods were tested on a blood sample of a healthy donor, resulting in good agreement with literature data. The only limitation of this approach is size detection limit, which is currently about 0.5 mu m due to used laser wavelength of 0.66 mu m. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.5.057006]
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