期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 17, 期 1, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.17.1.015004
关键词
optical clearing; glycerol; Raman microspectroscopy; porcine skin; frequency shift
资金
- National Natural Science Foundation of China [60778047]
- Natural Science Foundation of Guangdong Province [9251063101000009]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [200805740003]
In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved. (c) 2012 society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.1.015004]
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