期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 16, 期 2, 页码 -出版社
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3548878
关键词
poly (lactic-co-glycolic acid) (PLGA); PI3K/AKT inhibition; DNA demethylation; microsphere; epigenetic therapy; biotinylation; fluorescence imaging
资金
- National Institutes of Health [U54 CA113001, R01 CA 069065]
- Department of Defense [W81XWH-07]
- OSU Department of Radiology
We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K/AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3548878]
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