期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 16, 期 9, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3625288
关键词
fluorescence lifetime imaging microscopy; time-resolved imaging; photon counting; complementary metal-oxide-semiconductor; single-photon avalanche diode; center-of-mass
资金
- European Commission [HEALTH-F2-2009-222741, 029217-2]
- EPSRC
- Medical Research Council [G1000758, G1000758B] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0508-10212] Funding Source: researchfish
A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 x 32 low dark count 0.13 mu m complementary metal-oxide- semiconductor single-photon avalanche diode (SPAD) plus time-to-digital converter array. A simple look-up table is included to enhance the lifetime resolvability range and photon economics, making it comparable to the commonly used least-square method and maximum-likelihood estimation based software. To demonstrate its performance, a widefield microscope was adapted to accommodate the SPAD array and image different test samples. Fluorescence lifetime imaging microscopy on fluorescent beads in Rhodamine 6G at a frame rate of 50 fps is also shown. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3625288]
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