期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 15, 期 6, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3523616
关键词
fluorescence spectroscopy; turbid media; diffuse reflectance; cancer diagnosis; fluorescence quantification; tissue optics; protoporphyrin IX
资金
- National Institutes of Health [R01NS052274-01A1]
- Natural Sciences and Engineering Research Council of Canada
We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast. (C) 2010 society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3523616]
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