期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 14, 期 1, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3067726
关键词
hypericin (HY); N-methyl pyrrolidone (NMP); polyvinylpyrrolidone (PVP); fluorescence correlation spectroscopy (FCS); fluorescence lifetime imaging microscopy (FLIM); digitized fluorescence endoscopic imaging (DFEI); chick chorioallantoic membrane (CAM); photodynamic therapy and diagnosis; drug delivery
资金
- National University of Singapore [143-000-338-112]
We report the detection of interactions between a photosensitizer, hypericin (HY), and its solvent system prepared with a formulation additive, polyvinylpyrrolidone (PVP), a commonly used pharmaceutical excipient. Fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) were used to study aggregation and binding of HY in the presence of PVP. Digitized fluorescence endoscopic imaging (DFEI) was used to study the effect of the pharmaceutical formulation in the in vivo tumor implanted chick chorioallantoic membrane (CAM) model. The results presented reveal the coordination of HY-PVP binding, HY disaggregation in the presence of PVP, and strengthened HY tumor uptake selectivity. PVP is thus suggested as a potential adjuvant to previously investigated N-methyl pyrrolidone (NMP) in the HY delivery system as well as a replacement for the conventionally used albumin in the HY bladder instillation fluids preparation for clinical use.(c) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3067726]
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