期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 14, 期 5, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3213601
关键词
laser microbeam; spatially sculpted scissors; DNA damage
资金
- National Institutes of Health Laser Microbeam and Medical Program [RR01192]
- Air Force Office of Scientific Research [FA9550-04-10101]
- Beckman Laser Institute Inc. Foundation
- National Institutes of Health [CA100710]
- NATIONAL CANCER INSTITUTE [R01CA100710] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR001192] Funding Source: NIH RePORTER
We present a simple and efficient method for controlled linear induction of DNA damage in live cells. By passing a pulsed laser beam through a cylindrical lens prior to expansion, an elongated elliptical beam profile is created with the ability to expose controlled linear patterns while keeping the beam and the sample stationary. The length and orientation of the beam at the sample plane were reliably controlled by an adjustable aperture and rotation of the cylindrical lens, respectively. Localized immunostaining by the DNA double strand break (DSB) markers phosphorylated H2AX (gamma H2AX) and Nbs1 in the nuclei of HeLa cells exposed to the line scissors was shown via confocal imaging. The line scissors method proved more efficient than the scanning mirror and scanning stage methods at induction of DNA DSB damage with the added benefit of having a greater potential for high throughput applications. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3213601]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据