期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 9, 期 12, 页码 2063-2072出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2013.1764
关键词
Upconversion Nanoparticles; Poly(acrylicacid); pH-Responsive Drug Delivery; Doxorubicin; Upconversion Fluorescence Resonance Energy Transfer (UFRET) Imaging
资金
- Project of Natural Science Foundation of China [21175039, 21190044]
- Key Technologies Research and Development Program of China [2011AA02a114]
- Research Fund for the Doctoral Program of Higher Education of China [20110161110016]
- Hunan Provincial Natural Science Foundation
- Hunan Provincial Science and Technology Plan of China [10JJ7002, 2012TT1003]
A poly(acrylicacid)-modified NaYF4: Yb, Er upconversion nanoparticles (PAA-UCNPs) with dual functions of drug delivery and release imaging have been successfully developed. The PAA polymer coated on the surface of UCNPs serve as a pH-sensitive nanovalve for loading drug molecules via electrostatic interaction. The drug-loading efficiency of the PAA-UCNPs was investigated by using doxorubicin hydrochloride (DOX) as a model anticancer drug to evaluate their potential as a delivery system. Results showed loading and releasing of DOX from PAA-UCNPs were controlled by varying pH, with high encapsulation rate at weak alkaline conditions and an increased drug dissociation rate in acidic environment, which is favorable for construct a pH-responsive controlled drug delivery system. The in vitro cytotoxicity test using HeLa cell line indicated that the DOX loaded PAA-UCNPs (DOX@PAA-UCNPs) were distinctly cytotoxic to HeLa cells, while the PAA-UCNPs were highly biocompatible and suitable to use as drug carriers. Furthermore, the upconversion fluorescence resonance energy transfer (UFRET) imaging through the two-photon laser scanning microscopy (TLSM) revealed the time course of intracellular delivery of DOX from DOX@PAA-UCNPs. Thus, PAA-UCNPs are effective for constructing pH-responsive controlled drug delivery systems for multi-functional cancer therapy and imaging.
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