期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 9, 期 8, 页码 1378-1382出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2013.1633
关键词
Glutamate; Acetylcholinesterase (AChE); Electrochemical Biosensor; Nano-Porous Pseudo Carbon Paste Electrode (Nano-PPCPE); Neurotransmitter
资金
- National Key Program for Developing Basic Research of China [2010CB933903]
- NSFC [60971045, 21205013, 21205036, 61271056, 61201033]
- Hunan Science and Technology Projects [2012SK3105, 2010sk2003]
- Natural Science Foundation of Hunan Province [12JJ6060, 12JJ9038]
- Scientific Research Fund of Hunan Provincial Education Department [11A030]
- China Postdoctoral Science Foundation [2012M520980]
Using two nano-porous pseudo carbon paste electrodes (Nano-PPCPEs) as the working electrodes, a saturated calomel electrode as the reference electrode, and a Pt electrode as the counter electrode to form a four-electrode system, a novel type of glutamate-acetylcholine electrochemical biosensor array was successfully fabricated. It was shown that the biosensor array displayed a high selectivity and sensitivity and the selective and simultaneous determination of glutamate and acetylcholine (ATCh) is feasible. For L-glutamate, a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M was demonstrated, while for ATChl the linear range is from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M. This research means a possibility to simultaneously detect more neurotransmitters with a nano-PPCPEs electrochemical biosensor array.
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