期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 99B, 期 1, 页码 111-119出版社
WILEY
DOI: 10.1002/jbm.b.31877
关键词
degradation; cell-material interaction; toxicity; metal ions; corrosion
资金
- National Agency of Scientific and Technological Promotion (ANPCyT) [BID 1728 OC/AR PICT 0533225, PICT 05-32906, PAE 22771]
- National University of La Plata (UNLP) [11-I129]
- National Council for Scientific and Technological Research (CONICET)
Mg is a biodegradable biomaterial which may release particles (MP) to the environment. The possible cyto- and genotoxic effects of MP derived from magnesium powder (mesh 325) were analyzed on rat osteosarcoma UMR106 cells in order simulate the effect of Mg debris. Neutral red (NR) incorporation and acridine orange/ethidium bromide (AO/EB) staining techniques were used as endpoints to analyze the cytotoxic effects at 25-1000 mu g/mL concentration range. Genotoxicity was estimated according to micronucleus (MN) formation and the Comet assay (CA). Results showed that MP size changes with time due to corrosion. Changes in lysosomal activity were observed after 24 h only at 1000 mu g/mL. Accordingly, AO/EB staining showed a significant decrease in the number of living cells at 500 mu g/mL. Transmission electronic microscopy showed MP internalization (60 and 200 nm diameter) in cells after 2-h treatment, whereas no MP was detected after 24 h. A significant dose-dependent increase in MN frequencies was observed at 25-100 mu g/mL range (nontoxic range). DNA damage induction was assessed by CA only at 500 mu g/mL. Results showed dose-dependent cytotoxic and genotoxic effects of MP on UMR106 cells with different threshold values of MP concentration. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 99B: 111-119, 2011.
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