期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 95B, 期 1, 页码 29-35出版社
WILEY
DOI: 10.1002/jbm.b.31679
关键词
biodegradation; collagen; scaffolds; gamma-polyglutamic acid; cohesive plug
资金
- National Science Council, R.O.C [NSC-95-2221-E-182-010]
In the past decade, numerous studies have been devoted to developing natural bioadhesives that have the notable capacity to adhere to wet surfaces. Collagen and gamma-poly-glutamic acid (gamma-PGA) are well-known natural hydrophilic polymers that have both been utilized for their versatility in a wide range of biomedical applications. The aim of this study was the construction and characterization of a cohesive plug composed of gamma-PGA and reconstituted collagen fibrils crosslinked with water-soluble carbodiimide. Transmission electron microscopy examinations confirmed that the collagen fibrils in the reconstituted collagen/gamma-PGA gel retained their native specific D-period structure. This unique D-pattern structure of collagen plays a major role in hemostasis and is also related to several cellular behaviors. The bonding strength of the reconstituted collagen/gamma-PGA adhesive was approximately 42.9 +/- 4.0 KPa after 5 min of application and increased to 76.5 +/- 15.1 KPa after 24 h. This was much stronger than the fibrin adhesive, whose bonding strength was 30.9 +/- 0.2 KPa. Furthermore, the reconstituted collagen/gamma-PGA gel degraded gradually after subcutaneous implantation in the backs of rats over a period of 8 weeks, without any severe inflammatory response. On the basis of the histological analysis, fibroblasts migrated into the gel while it degraded, which indicates that the gel is not harmful to cellular activity. Together, these findings demonstrate that using reconstituted collagen with retained D-periodicity as a component of the bioadhesive is a possibly better option to formulate effective adhesiveness and is promising as a scaffold for tissue repair. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 958: 29-35, 2010.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据