期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 103, 期 5, 页码 1659-1668出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.35293
关键词
adhesion; cell migration; microstructures; focal adhesion kinase; cell morphology
资金
- Spanish Ministry of Education, FPU grant system
- BBSRC
- EPSRC
- CIBER-BBN VI National RDi Plan
- Iniciativa Ingenio
- Consolider Program
- Instituto de Salud Carlos III
- European Regional Development Fund
- Commission for Universities and Research of the Department of Innovation, Universities
- Fundacion M. Botin, Santander, Spain
- Enterprise of the Generalitat de Catalunya [2009 SGR 505]
- Biotechnology and Biological Sciences Research Council [BB/D015324/1] Funding Source: researchfish
- British Heart Foundation [PG/15/15/31316] Funding Source: researchfish
- BBSRC [BB/G008868/1, BB/D015324/1] Funding Source: UKRI
- EPSRC [EP/K034898/1, EP/G048703/1] Funding Source: UKRI
It is known that cells respond strongly to microtopography. However, cellular mechanisms of response are unclear. Here, we study wild-type fibroblasts responding to 25 mu m(2) posts and compare their response to that of FAK(-/-) fibroblasts and fibroblasts with PMA treatment to stimulate protein kinase C (PKC) and the small g-protein Rac. FAK knockout cells modulated adhesion number and size in a similar way to cells on topography; that is, they used more, smaller adhesions, but migration was almost completely stalled demonstrating the importance of FAK signaling in contact guidance and adhesion turnover. Little similarity, however, was observed to PKC stimulated cells and cells on the topography. Interestingly, with PKC stimulation the cell nuclei became highly deformable bringing focus on these surfaces to the study of metastasis. Surfaces that aid the study of cellular migration are important in developing understanding of mechanisms of wound healing and repair in aligned tissues such as ligament and tendon. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1659-1668, 2015.
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