期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 102, 期 6, 页码 1982-1990出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34873
关键词
methacrylamide-modified gelatin; hydrogel; polyimide implant; immunocompatibility; subcutaneous implantation
资金
- Research Foundation - Flanders (FWO)
- Czech Science Foundation [P301/12/1254]
- RVO [61388971]
- EC (EU-STREP) [019114]
Immunocompatibility of gelatin-based hydrogels to be applied as implant coatings for local regenerative treatment has been studied. First, the bio- and immuno-acceptability of the methacrylamide-modified gelatin hydrogels per se was screened. The results indicated that the hydrogels support cell growth. Metabolic activity of normal cells and permanent cell lines representing various cell types (endothelial, epithelial, fibroblast, and monocyte/macrophage) cultivated on the gelatin hydrogels was moderately lower compared to cells cultivated on tissue culture plastic. The cells cultivated on the hydrogels produced identical cytokines as the control cells although at lower levels. Importantly, no inflammatory activity, measured by nitric oxide and pro-inflammatory cytokine (IL-1, IL-6, and TNF) production, was observed in peritoneal cells and monocyte/macrophage RAW 264.7 cell line cultivated on the hydrogels. Finally, polyimide (PI) implantable membranes were surface-modified with gelatin hydrogels and screened for their in vivo immunocompatibility. Their histological examination performed after subcutaneous implantation in mice produced a sound proof of immunoacceptability. Normal tissue repair, mild cellular infiltration and edema mainly induced by the surgery were observed after 2 and 6 days. No adverse tissue responses were induced by the implants. Analysis performed after 4 and 9 weeks indicated areas of foreign body granuloma without formation of a fibrous capsule. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1982-1990, 2014.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据