期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 102, 期 4, 页码 1102-1109出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34776
关键词
polydopamine; alkaline phosphatase; enzyme immobilization; surface modification; titanium implants
资金
- Royal Netherlands Academy of Arts and Sciences (KNAW), Programme Strategic Scientific Alliances (PSA) [08-PSAM-02]
Immobilization of biomolecules onto implant surfaces is one of the most straightforward strategies to control the interaction between an implant and its biological environment. Recently, it was shown that the enzyme alkaline phosphatase (ALP) could be efficiently immobilized onto titanium implants in a single step using polydopamine. We hypothesized that such polydopamine-ALP coatings can enhance the early attachment of cells and increase mineralization. Therefore, the current study aimed at immobilization of ALP onto titanium by means of either one- or two-step polydopamine-assisted immobilization or electrospray deposition, the comparative characterization of these experimental substrates and subsequent cell behavioral analysis using primary osteoblast-like cells. Uncoated titanium and ALP-free polydopamine coatings served as controls. Despite significant ALP surface activity and lower water contact for angles ALP-containing surface modifications, only marginal effects on early cell behavior (i.e., cell spreading) and osteogenic differentiation (i.e., proliferation, differentiation and mineralization) were observed in comparison to uncoated titanium. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1102-1109, 2014.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据