期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 100A, 期 4, 页码 1033-1043出版社
WILEY
DOI: 10.1002/jbm.a.34053
关键词
silver nanoparticles; cell toxicity; in vitro; size dependence; endocytosis
资金
- Priority Research Centers Program [2009-0093829]
- WCU (World Class University) through National Research Foundation (NRF), Ministry of Education, Science and Technology [R31-10069]
Silver nanoparticles (AgNPs) have found a variety of uses including biomedical materials; however, studies of the cytotoxicity of AgNPs by size effects are only in the beginning stage. In this study, we examined the size-dependent cellular toxicity of AgNPs using three different characteristic sizes (similar to 10, 50, and 100 nm) against several cell lines including MC3T3-E1 and PC12. The cytotoxic effect determined based on the cell viability, intracellular reactive oxygen species generation, lactate dehydrogenase release, ultrastructural changes in cell morphology, and upregulation of stress-related genes (ho-1 and MMP-3) was fairly size- and dose-dependent. In particular, AgNPs stimulated apoptosis in the MC3T3-E1 cells, but induced necrotic cell death in the PC12 cells. Furthermore, the smallest sized AgNPs (10 nm size) had a greater ability to induce apoptosis in the MC3T3-E1 cells than the other sized AgNPs (50 and 100 nm). These data suggest that the AgNPs-induced cytotoxic effects against tissue cells are particle size-dependent, and thus, the particle size needs careful consideration in the design of the nanoparticles for biomedical uses. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
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